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目的 :探讨分泌性中耳炎渗出液中细胞因子的水平及其炎症的调节机制。方法 :用 EL ISA法检测本病患耳的渗出液中肿瘤坏死因子 -α(TNF-α)和可溶性肿瘤坏死因子受体 (TNFsol R )水平 ,分析其与临床指标间的关系。结果 :渗出液中 TNF- α和 TNFsol R 的检出率均为 10 0 % ;TNF- α值为 (3.42± 2 .2 5 ) ng/ g总蛋白 ,TNFsol R 为 (4 0 5 .80± 2 16 .5 3) ng/ g总蛋白 ,TNFsol R 指数为 (15 4.18± 90 .45 ) U;多次发病的患者 TNFsol R 和 TNFsol R 指数均降低 (P <0 .0 5 )。结论 :细胞因子 TNF- α和 TNFsol R 存在于所有分泌性中耳炎渗出液中 ,是此炎症反应的重要递质。复发的分泌性中耳炎渗出液中 TNFsol R 和 TNFsol R 指数明显降低 (P <0 .0 5 ) ,而 TNF- α无明显变化。
Objective: To investigate the level of cytokines in exudate of secretory otitis media and its mechanism of regulation of inflammation. Methods: The levels of tumor necrosis factor-α (TNF-α) and soluble tumor necrosis factor receptor (TNFsol R) in exudates of the patients were detected by EL-ISA, and their relationships with clinical parameters were analyzed. Results: The detection rates of TNF-α and TNFsol R in exudate were 100%, TNF-α was (3.42 ± 2.52) ng / g total protein, TNFsol R was (40.0580 ± 2 16.53) ng / g total protein. The TNFsol R index was (15 4.18 ± 90. 45) U. The TNFsol R and TNFsol R index of patients with multiple morbidity decreased (P <0.05). CONCLUSION: The cytokines TNF-α and TNFsol R are present in all exudative otitis media exudates and are important mediators of this inflammatory response. TNF secretin and TNFsol R index in the recurrent secretory otitis media excretion was significantly lower (P <0.05), while there was no significant change in TNF-α.