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目的端粒重复序列结合蛋白质1(telomeric-repeat binding factor-1,TRF1)和端粒重复序列结合蛋白质2(telomeric-repeat binding factor-2,TRF2)基因表达异常在原发性干燥综合征(primary Sjogren’s,pSS)发病中可能的作用。方法采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测55例pSS患者和41例健康对照者(healthy control,HC)外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中TRF1和TRF2转录水平,并与临床指标进行相关性分析。结果 (1)PBMCs TRF2转录水平在SS组显著高于HC组(0.0047±0.0107 vs.0.0026±0.0049,P=0.001),而TRF1转录水平在两组表达差异无统计学意义(P>0.05)。(2)Spearman相关性分析发现在SS组中TRF2与白细胞计数(WBC)(r=0.309,P<0.05)、中性粒细胞计数(GR)(r=0.312,P<0.05)、补体3(C3)(r=0.470,P<0.05)、抗核抗体(ANA)(r=0.339,P<0.05)呈显著正相关,TRF1转录水平与TRF2(r=1,P<0.01)、WBC(r=0.316,P<0.05)、GR(r=0.313,P<0.05)、ANA(r=0.421,P<0.05)呈显著正相关。结论 TRF2转录水平在SS患者的增高提示其可能参与了SS患者端粒酶激活和端粒缩短的调节;TRF2、TRF1表达水平与WBC、GR、C3、ANA呈显著正相关,提示TRF2、TRF1可能参与了SS炎症免疫调节,且与SS的发病机制有关。加强端粒保护蛋白在SS等自身免疫性疾病的研究有利于进一步阐明这类疾病的发病机制。
Objective To investigate the aberrant gene expression of telomeric-repeat binding factor-1 (TRF1) and telomeric-repeat binding factor-2 (TRF2) genes in patients with primary Sjogren’s syndrome Sjogren’s, pSS) in the pathogenesis of possible role. Methods 55 cases of pSS and 41 healthy control (HC) peripheral blood mononuclear cells (PBMCs) were detected by real-time quantitative polymerase chain reaction (RT-qPCR) PBMCs) TRF1 and TRF2 transcription levels, and clinical data and correlation analysis. Results (1) The transcription level of TRF2 in PBMCs in SS group was significantly higher than that in HC group (0.0047 ± 0.0107 vs.00.0026 ± 0.0049, P = 0.001). There was no significant difference in TRF1 transcription between the two groups (P> 0.05). (2) Spearman correlation analysis showed that there were significant differences in TRF2 and WBC (r = 0.309, P <0.05), neutrophil count (GR) C3 (r = 0.470, P <0.05) and ANA (r = 0.339, P <0.05). There was a significant positive correlation between TRF1 and TRF2 (r = = 0.316, P <0.05), GR (r = 0.313, P <0.05), ANA (r = 0.421, P <0.05). Conclusion The increase of TRF2 transcription level in patients with SS suggests that it may be involved in the regulation of telomerase activation and telomere shortening in SS patients. The expression levels of TRF2 and TRF1 are positively correlated with WBC, GR, C3 and ANA, suggesting that TRF2 and TRF1 may be involved Participated in SS inflammatory immune regulation, and the pathogenesis of SS. Strengthening the study of telomere protective proteins in SS and other autoimmune diseases is helpful to further elucidate the pathogenesis of these diseases.