以新疆野苹果[Malus sieversii(Ledeb.)Roem.]60份初级核心种质为试材,采用不同遗传距离构建新疆野苹果核心种质,利用数量性状参数均值差异百分率(MD)、方差差异百分率(VD)、极差符合率(CR)、变异系数变化率(VR)和分子标记参数多态位点百分率(p)、平均有效等位基因数(MNe)、平均多态信息含量(MPIC)和平均Shannon’s信息指数(MI)等8个指标评价不同方法构建核心种质的优劣,用选出的合适方法构建新疆野苹果核心种质。研究结果表明:利用混合遗传距离(Dmix)构建的核心种质整合了两类不同数据,优于单独使用农艺性状表型值数据或分子标记数据构建的核心种质,其VD为50%,CR为96.56%,VR为117.98%,p为97.67%,MNe为97.15%,MPIC为101.88%,MI为100.44%;利用15个农艺性状检测表明,所构建的42份新疆野苹果核心种质保留了300份原始种质93%以上的农艺性状,很好地代表了原始种质的遗传多样性。 Sixty primary core collections of Xinjiang Malus sieversii (Ledeb.) Roem. Were used as materials to construct core collection of Xinjiang apple. The average percentage difference (VD), CR, CR, percentage of polymorphic loci (M), average polymorphic information content (MPIC) And Shannon’s Information Index (MI) were used to evaluate the advantages and disadvantages of different methods to construct core collection. The core collection of Xinjiang apple was constructed by the appropriate selection method. The results showed that the core collection constructed by using Dmix integrated two types of data, which was better than the core collection constructed using agronomic trait phenotype data or molecular marker data alone, with a VD of 50%, CR Of which were 96.56%, VR was 117.98%, p was 97.67%, MNe was 97.15%, MPIC was 101.88% and MI was 100.44% .Through agronomic traits tests, 42 core apple germplasms preserved The agronomic traits of more than 93% of the original germplasm of 300 were representative of the genetic diversity of the original germplasm.