Dislocation behaviour of a twinning-induced plasticity (TWIP) steel subjected to high-cycle fatigue tests is investigated in the present study.Grain boundaries are the important sources of dislocation generation during fatigue tests,contributing to the increase in dislocation density.Continuous emission of dislocations from grain boundaries is observed in many grains.Inclusions can sustain large dislocation pile-ups at the inclusion interfaces,leading to a high stress concentration and therefore acting as potential sites of microcrack nucleation.In contrast,annealing twin boundaries are relatively weak boundaries for dislocation pile-ups.When the number of dislocations in a pile-up is large,dislocations can crossover twin boundaries and glide inside the annealing twins.The stress concentration at the twin boundary is relatively low so that twin boundaries could not act as the sites for microcrack initiation.