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通过不同浓度葡聚六糖和接种立枯病菌处理,研究了葡聚六糖对菜薹接种立枯病菌后的发病率、病情相关蛋白(PRs)和内源激素含量的影响。结果表明:葡聚六糖处理可提高菜薹对立枯病菌的抗性,降低发病率。葡聚六糖处理对菜薹产量没有明显的影响,而接种立枯病菌后,产量明显降低,但葡聚六糖处理可以抑制立枯病菌对菜薹生长的影响,减轻产量的损失。葡聚六糖和立枯病菌处理都能明显诱导提高菜薹植株体内病情相关蛋白几丁质酶、β–1,3–葡聚糖酶和内源激素乙烯、吲哚乙酸(IAA)、脱落酸(ABA)的水平,但葡聚六糖的诱导作用明显大于立枯病菌。这些结果表明,葡聚六糖可提高植株体内病情相关蛋白几丁质酶和β–1,3–葡聚糖酶的活性,通过较高水平的内源激素乙烯、IAA和ABA的代谢传递抗病信号,诱导菜薹植株对立枯病的系统抗性。
The effects of glucohexaose on the incidence, disease-related proteins (PRs) and endogenous hormones of Brassica juncea after inoculation with Brassica campestris were studied by treatment with different concentrations of glucohexasaccharide and Rhizoctonia solani. The results showed that glucanotetraose treatment could increase the resistance of cabbage to Rhizoctonia solani and reduce the incidence. Glucohexaose treatment had no obvious effect on the yield of flowering cabbage, but the yield was significantly decreased after inoculation with R.solani. However, glucohexaose treatment could inhibit the influence of R. solani on the growth of cabbage and reduce the loss of yield. Both glucohexasaccharide and Rhizoctonia solani significantly induced the increase of disease-related proteins chitinase, β-1,3-glucanase and endogenous hormones ethylene, indoleacetic acid (IAA) Acid (ABA) levels, but the inducing effect of glucohexaose was significantly higher than that of Rhizoctonia solani. These results indicate that glucohexaose enhances the activity of disease-associated proteins chitinase and β-1, 3-glucanase in plants through the metabolism of higher endogenous hormones ethylene, IAA and ABA Systematic resistance of rape plants to blight was induced by disease signals.