应用红外光谱分析法（ＩＲ）研究了醋酸锌与胆固醇的相互作用，表明胆固醇的羟基吸收峰没有改变，从而判定锌（Ⅱ）与胆固醇之间难于进行化学反应；以分光光度法（ＳＡ）、原子吸收光谱分析法（ＡＡＳ）和电感耦合等离子体原子发射光谱法（ＩＣＰＡＥＳ）考察了水溶液中锌（Ⅱ）与胆固醇的相互作用．结果表明，锌（Ⅱ）与胆固醇之间存在表面吸附作用．讨论了这种作用的吸附特征，并证明在近生理条件下较为显著． The interaction between zinc acetate and cholesterol was studied by infrared spectroscopy (IR), which showed that the hydroxyl absorption peak of cholesterol did not change, which made it difficult to determine the chemical reaction between zinc (Ⅱ) and cholesterol. Spectrophotometry (SA) Atomic Absorption Spectrometry (AAS) and Inductively Coupled Plasma Atomic Emission Spectrometry (ICPAES) were used to investigate the interaction between zinc (Ⅱ) and cholesterol in aqueous solution. The results show that there is surface adsorption between zinc (Ⅱ) and cholesterol. The adsorption characteristics of this effect are discussed and demonstrated to be more significant under near physiological conditions.