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目的研究miRNA-30b纳米粒对甲状腺未分化癌细胞株TA-K细胞的生物学特性的影响。方法转染不同浓度的miRNA-30b纳米粒进入TA-K细胞中,通过流式法检测TA-K细胞凋亡情况,后通过生物信息学预测miRNA-30b的生物学靶点,并通过RT-PCR及Western Blot方法验证。结果发现转染了miRNA-30b纳米粒后,TA-K细胞的凋亡显著增加,而生物信息学显示miRNA-30b可靶向作用于Survivin,后经RT-PCR及Western blot法证实Survivin确实是miRNA-30b的生物学靶点。结论 miRNA-30b可以通过作用Survivin促进TA-K细胞的凋亡,这一现象也为将来甲状腺未分化癌的临床治疗提供了新的思路。
Objective To investigate the effect of miRNA-30b nanoparticles on the biological characteristics of TA-K cells in thyroid undifferentiated carcinoma cell line. Methods The different concentrations of miRNA-30b nanoparticles were transfected into TA-K cells. The apoptosis of TA-K cells was detected by flow cytometry. The biological targets of miRNA-30b were predicted by bioinformatics. PCR and Western Blot method validation. The results showed that the apoptosis of TA-K cells was significantly increased after transfection of miRNA-30b nanoparticles, and bioinformatics showed that miRNA-30b could target Survivin, and confirmed by RT-PCR and Western blot that Survivin indeed The biological target of miRNA-30b. Conclusions miRNA-30b can promote the apoptosis of TA-K cells through the action of Survivin. This phenomenon also provides a new idea for the future clinical treatment of undifferentiated thyroid carcinoma.