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将低分子量壳聚糖与茶多酚复配,制成壳聚糖与茶多酚复配物,并采用紫外可见光分光光度计研究了该复配物对超氧阴离子自由基、羟自由基、过氧化氢和1,1-二苯基-2-苦肼基自由基(DPPH)的清除能力。结果表明,壳聚糖与茶多酚复配物的超氧阴离子自由基、羟自由基、过氧化氢和DPPH的清除能力均高于单独壳聚糖,并且随着复配物中茶多酚含量增加清除率增大,表明复配物的抗氧化性高于壳聚糖;当V(壳聚糖)︰V(茶多酚)为6︰4~2︰8时,复配物的羟自由基清除率高于单独壳聚糖或茶多酚,在V(壳聚糖)︰V(茶多酚)为2︰8时复配物DPPH清除率比茶多酚提高0.70%、比壳聚糖提高6.67%,表明壳聚糖与茶多酚之间存在协同增效作用。
The low molecular weight chitosan compound with tea polyphenols, made of chitosan and tea polyphenols compound, and the use of UV-visible spectrophotometer study of the complex superoxide anion free radicals, hydroxyl free radicals, Hydrogen peroxide and 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) scavenging capacity. The results showed that the scavenging abilities of superoxide anion free radical, hydroxyl radical, hydrogen peroxide and DPPH of chitosan and tea polyphenol complex were higher than that of chitosan alone. With the increase of tea polyphenol When the content of V (chitosan): V (polyphenols) was 6︰4 ~ 2︰8, the hydroxyl groups of the complex Free radical scavenging rate was higher than that of chitosan or polyphenols alone. When the ratio of V (chitosan): V (polyphenols) was 2︰8, the scavenging capacity of DPPH was 0.70% higher than that of tea polyphenols, Glycan increased 6.67%, indicating that there is synergy between chitosan and tea polyphenols.