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目的:探讨原发性头颈部鳞癌ＣＤ４４Ｖ６、Ｐ５３及ＰＣＮＡ蛋白的表达及临床意义。方法:采用ＳＰ免疫组化染色方法,检测８０例头颈部鳞癌ＣＤ４４Ｖ６、ｐ５３及ＰＣＮＡ蛋白的表达。结果:①头颈部鳞癌ＣＤ４４Ｖ６表达阳性率为３１．３%,淋巴结转移组阳性率显著低于无淋巴结转移组(Ｐ ＜０．０５),低分化癌组阳性表达率低于高分化癌组(Ｐ＜０．０５);②Ｐ５３蛋白在淋巴结转移组阳性率显著高于无淋巴结转移组(Ｐ＜０．０１),低分化癌组阳性率高于高分化癌组(Ｐ＜０．０１);③ＰＣＮＡ强阳性表达在淋巴结转移、低分化癌与无淋巴结转移、高分化癌均有显著差异(Ｐ＜０．０１)。结论:头颈部鳞癌 ＣＤ４４Ｖ６低度表达、Ｐ５３及ＰＣＮＡ蛋白的高表达,提示肿瘤恶性度高、易发生淋巴结转移。
Objective: To investigate the expression of CD44V6, P53 and PCNA protein in primary head and neck squamous cell carcinoma and its clinical significance. Methods: SP immunohistochemical method was used to detect the expression of CD44V6, p53 and PCNA protein in 80 cases of head and neck squamous cell carcinoma. Results: ① The positive rate of CD44V6 expression in head and neck squamous cell carcinoma was 31.3%, the positive rate in lymph node metastasis was significantly lower than that in non-lymph node metastasis (P <0.05), but the positive rate in poorly differentiated carcinoma was lower than that in well differentiated carcinoma (P <0.05). The positive rate of P53 protein in lymph node metastasis group was significantly higher than that in non-lymph node metastasis group (P <0.01), and the positive rate in poorly differentiated carcinoma was higher than that in well differentiated carcinoma (P <0.01) ); ③PCNA strong positive expression in lymph node metastasis, poorly differentiated carcinoma and no lymph node metastasis, well differentiated carcinoma were significantly different (P <0.01). Conclusion: Low expression of CD44V6 and high expression of P53 and PCNA in squamous cell carcinoma of the head and neck suggest that the tumor is highly malignant and prone to lymph node metastasis.