目的　针对柯萨奇病毒 3型 (CVB3)基因组重要功能区设计系列反义寡聚核苷酸序列 ,通过实验观察特异性反义核酸对病毒感染细胞的抑制作用。方法　通过病毒空斑形成试验及致细胞病变作用 ,了解反义核酸抑制及阻断CVB3 病毒感染细胞的能力。结果　实验所设计的反义核酸中SCB1对感染HeLa细胞的CVB3 病毒活性抑制作用较强 ,当病毒感染量为 1MOI时 ,病毒活性抑制率高达 90 % ;SCB4 和SCB2 均为 75 % ;SCB6有 5 0 %抑制作用 ;而SCB3 和SCB5以及非特异性对照序列对CVB3病毒基因基本没有任何作用。结论　通过以上实验确定SCB1、SCB2 、SCB4 等较好的抗病毒反义核酸序列 ,为进一步研究反义核酸抗病毒作用提供了重要的实验基础 OBJECTIVE: To design a series of antisense oligonucleotide sequences targeting the important functional regions of Coxsackievirus 3 (CVB3) genome and to observe the inhibitory effect of specific antisense nucleic acids on virus-infected cells. Methods The virus plaque formation assay and cytopathic effect were used to understand the ability of antisense oligonucleotides to inhibit and block CVB3-infected cells. Results The antisense nucleic acids designed in this study showed that SCB1 had a strong inhibitory effect on the activity of CVB3 in HeLa cells. When the viral load was 1 MOI, the inhibition rate of virus activity was as high as 90%; SCB4 and SCB2 were 75%; SCB6 was 5 0% inhibition; while SCB3 and SCB5 as well as non-specific control sequences of the CVB3 virus gene had no effect at all. Conclusion The better antiviral antisense nucleic acid sequences such as SCB1, SCB2 and SCB4 were determined by the above experiments, which provided an important experimental basis for further study on the anti-virus effect of antisense nucleic acids