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目的 :探讨复方保肝宁对HSC -T6细胞增殖及Ⅰ型胶原表达的影响。方法 :复方保肝宁给正常大鼠灌胃 ,制备药物血清 ,温育培养HSC -T6细胞 4 8小时。MTT法测定星状细胞增殖 ,ELISA法测定培养上清的Ⅰ型胶原表达量。结果 :复方保肝宁药物血清能明显抑制HSC -T6细胞增殖 ,抑制细胞的Ⅰ型胶原分泌。实验表明 ,在抑制HSC -T6细胞增殖和Ⅰ型胶原蛋白分泌方面 ,高剂量组药物血清无低、中剂量组明显 ,是否为血清药物浓度对细胞的影响 ,尚需进一步研究。结论 :复方保肝宁能明显抑制HSC -T6细胞的活化 ,对HSC -T6细胞Ⅰ型胶原表达的抑制可能是该方抗肝纤维化的主要作用机制之一。
Objective : To explore the effect of compound Baoganing on the proliferation of HSC-T6 cells and the expression of type I collagen. METHODS: Compound Baoganning was administered to normal rats by gavage, and drug serum was prepared. HSC-T6 cells were incubated for 48 hours. The proliferation of stellate cells was measured by MTT assay. The expression of type I collagen in culture supernatants was determined by ELISA. Results: The serum of compound Baoganing could obviously inhibit the proliferation of HSC-T6 cells and inhibit the secretion of type I collagen in cells. Experiments show that, in the inhibition of HSC-T6 cell proliferation and type I collagen secretion, high-dose group drug serum is not low, medium dose group was obvious, whether the serum drug concentration on the cell, need further study. Conclusion: Compound Baoganning can obviously inhibit the activation of HSC-T6 cells. The inhibition of type I collagen expression in HSC-T6 cells may be one of the main mechanisms of the anti-hepatic fibrosis.