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本文研究了大豆萌发过程中活性氧的产生与清除，并探讨了光因子在活性氧代谢中的作用。大豆呼吸强度、O产生速率及H2O2水平都在吸水后第四天达到高峰，然后下降，三者的变化趋势同步。SOD、POD及APX的活性随萌发过程而逐渐增强，最后趋于平稳。SOD同工酶谱中分别于萌发的第二、第三天各出现一条新的酶带。CAT在萌发的初期猛增50倍左右，之后趋于稳定。在三种清除H2O2的酶（CAT、POD、APX）中，CAT清除H2O2的能力远远高于POD与APX，CAT可能是大豆萌发过程中最主要的H2O2清除酶。光萌发时呼吸强度低于暗中萌发，但O产生速率与H2O2水平高于暗萌发，光萌发时O的产生占总耗氧量的1．1—2．7％，而暗中萌发为0．9—1．3％。光条件下SOD、APX活性明显高于暗中萌发，而POD与CAT则在光和暗条件下相差不大。
In this paper, the production and removal of reactive oxygen species during the germination of soybean were studied, and the role of photon in the active oxygen metabolism was also discussed. Soybean respiration intensity, O production rate and H2O2 level all reached its peak on the fourth day after water absorption, and then decreased. The trend of the three was synchronous. The activities of SOD, POD and APX gradually increased with the germination process and finally stabilized. SOD isozymes were germinated in the second and third days of each appeared a new enzyme band. CAT in the germination of the early surge 50 times, then tends to be stable. Among the three H2O2-removing enzymes (CAT, POD and APX), the ability of CAT to remove H2O2 was much higher than that of POD and APX. CAT was probably the most important H2O2 scavenger in soybean germination. The light intensity of germination was lower than that of dark germination, but the O production rate and H2O2 level were higher than that of dark germination. The light production of O accounted for 1.1-2.7% of the total oxygen consumption while the dark germination was 0.9 -1.3%. Under light conditions, the activities of SOD and APX were significantly higher than those in the dark, while POD and CAT did not change much under the light and dark conditions.