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目的通过研究活化增视颗粒对兔眼实验性增殖性玻璃体视网膜病变(PVR)玻璃体中MCP-1以及TGF-β2的影响,探讨活化增视颗粒对PVR的作用机制。方法将45只新西兰大白兔随机平均分为模型对照组、沃丽汀组、活化增视颗粒组,采用兔眼玻璃体内注射巨噬细胞的方法建立PVR的动物模型,各组于3、7、14、21、28 d分别随机处死3只,ELISA检测玻璃体液中MCP-1和TGF-β2的质量浓度。结果活化增视颗粒组在7 d时可显著降低兔玻璃体内MCP-1表达,与沃丽汀组及对照组相比差异有统计学意义(P<0.01),活化增视颗粒组在3 d时玻璃体内TGF-β2质量浓度开始升高,与对照组相比差异有统计学意义(P<0.01),与沃丽汀组相比差异无统计学意义(P>0.05),至14 d时达到高峰,与对照组及沃丽汀组相比差异有统计学意义(P<0.01)。结论活化增视颗粒主要在PVR炎症期及增生早期通过降低玻璃体中MCP-1和升高玻璃体中TGF-β2的质量浓度来抑制PVR的发生。
OBJECTIVE: To investigate the effect of Activated Zengshen Granules on MCP-1 and TGF-β2 in the vitreous of experimental proliferative vitreoretinopathy (PVR) in rabbits and to explore the mechanism of activating Zengshen Granules on PVR. Methods Forty five New Zealand white rabbits were randomly divided into model control group, Wolimin group and Activation Zengshen Granule group. Animal models of PVR were established by intravitreal injection of macrophages in rabbits. Three rabbits were randomly sacrificed at 14, 21 and 28 days respectively. The concentrations of MCP-1 and TGF-β2 in vitreous humor were determined by ELISA. Results Activating Zengshen granule group could significantly reduce the expression of MCP-1 in vitreous of rabbits at 7 days, which was significantly different from that of the control group (P0.01) (P <0.01). There was no significant difference between the two groups (P> 0.05). When the concentration of TGF-β2 in vitreous began to increase, the difference was statistically significant compared with the control group Reaching the peak, compared with the control group and the Wollitin group, the difference was statistically significant (P <0.01). Conclusion Activating Zengshi Granules can inhibit the occurrence of PVR mainly through decreasing the concentration of MCP-1 in the vitreous and increasing the concentration of TGF-β2 in the vitronectin in the early stage of PVR inflammation and PVR.