论文部分内容阅读
目的:通过对人遗传性骨软骨瘤软骨细胞的体外培养,观察肿瘤软骨细胞的生长特性及相关生物学特点,为进一步研究骨软骨瘤细胞学起因奠定基础。方法:原代培养遗传性骨软骨瘤组织中的软骨细胞并进行传代,进行活细胞形态观察,同时测定细胞增殖能力及贴壁率,与正常人关节软骨细胞做对照。结果:原代细胞在经过完全消化后,细胞活力仍达到95%。传代后骨软骨瘤细胞体积增大,细胞呈现星形,树突增多,细胞内颗粒多且明显。骨软骨瘤细胞增殖随着传代的增加减慢,第2代细胞增殖能力已经与原代细胞产生明显差别(P<0.01,t=3.203)。原代骨软骨瘤细胞在24h内大部分贴壁,随着传代的增加,细胞贴附能力下降,第4代细胞贴壁率与原代细胞产生明显区别(P<0.01,t=4.611)。结论:体外培养的骨软骨瘤细胞从形态,生物学特性(细胞增殖情况、细胞贴附能力)均不同于正常软骨细胞,为今后更加深入的研究遗传骨软骨瘤的病因学奠定一定的体外培养基础。
OBJECTIVE: To observe the growth characteristics of tumor chondrocytes and related biological characteristics of human hereditary osteochondroma chondrocytes in vitro and to lay the foundation for further study of osteochondroma cytology. Methods: Primary chondrocytes in hereditary osteochondroma were cultured and passaged, and morphological observation of living cells was performed. The proliferation and attachment rate of cells were also measured, and compared with normal human articular chondrocytes. Results: After complete digestion of primary cells, cell viability still reached 95%. After passage osteochondroma cells increased in size, the cells showed a star, dendritic increased, more intracellular particles and obvious. The proliferation of osteochondroma cells decreased with the increase of passage, the proliferation ability of the second generation cells had a significant difference with the primary cells (P <0.01, t = 3.203). Primary osteochondroma cells adhered most of the cells within 24 hours. With the passage of time increasing, cell attachment ability decreased. The attachment rate of the 4th generation cells was significantly different from that of primary cells (P <0.01, t = 4.611). CONCLUSION: The osteochondroma cells cultured in vitro are different from the normal chondrocytes in morphological and biological characteristics (cell proliferation and cell attachment ability) and lay a solid foundation for the further study of the etiology of hereditary osteochondroma in the future basis.