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通过冷休克的方法诱导红鳍东方鲀的受精卵,孵化后的仔鱼,用PAS-Ⅲ型细胞流速仪测定其倍性,得到较高的三倍体诱导率。受精后8 min,0℃下处理15 min和受精后5 min,2℃下处理15min三倍体率最高,为100%;受精后8 min,4℃下处理10 min倍化率最低,为20%;其余几组的三倍体率为90%。正交试验结果表明,影响三倍体诱导率的主次顺序是处理时间、处理温度、处理起始时间。
The cold fertilization method was used to induce the fertilized eggs and the hatched larvae of Picea obovata, and the ploidy of the larvae was determined by PAS-Ⅲ cell flow cytometer to obtain high triploid induction rate. After fertilization for 8 min, 0 ℃ for 15 min and 5 min after fertilization, 2 h at 15 ℃, the highest triploid rate was 100%, and the rate of doubling was the lowest at 10 min after 8 min of fertilization %; The other groups of triploid rate was 90%. The results of orthogonal test showed that the order of the primary and secondary factors that affect the induction rate of triploidy is the treatment time, the treatment temperature and the treatment start time.