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目的:建立简便有效的测定尿中N-乙酰-S-(N-甲基甲氨酰)半胱氨酸(AMCC)的高效液相色谱方法。方法:尿样经固相萃取后用高效液相色谱法分析,采用C18柱,以5%乙腈水溶液为流动相,流速1.0 ml/min,检测波长为196 nm。结果:尿中AMCC在1.00 mg/L～50.00 mg/L浓度范围内线性关系良好,相关系数为1.0000。方法精密度为2.8%～4.0%,检出限为0.1 mg/L,加样回收率为86.2%～96.1%。结论:该方法各项性能指标均符合职业卫生标准制定指南第5部分:生物材料中化学物质测定方法中相关要求,可用于测定二甲基甲酰胺(DMF)暴露工人尿中的AMCC含量。
Objective: To establish a simple and effective HPLC method for the determination of N-acetyl-S- (N-methylcarbamyl) cysteine (AMCC) in urine. Methods: Urine samples were analyzed by high performance liquid chromatography after solid-phase extraction. The mobile phase consisted of C18 column and 5% acetonitrile in water with a flow rate of 1.0 ml / min and a detection wavelength of 196 nm. Results: The urinary AMCC had a good linearity in the concentration range of 1.00 mg / L ~ 50.00 mg / L with a correlation coefficient of 1.0000. The precision of the method was 2.8% ~ 4.0%, the detection limit was 0.1 mg / L and the recoveries were 86.2% ~ 96.1%. Conclusion: All the performance indexes of this method are in accordance with the relevant requirements in the method of determination of chemical substances in biomaterials, Part 5 of the Guide for Occupational Health Standard: It can be used to determine the AMCC content in the urine of workers exposed to dimethylformamide (DMF).