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为探讨人胎肝中干细胞的存在并对其生物学特性进行鉴定 ,采用原代分离法培养人胎肝细胞集落 ,免疫组化鉴定细胞集落分子标志物的表达。结果 :从人胎肝组织中成功分离表达甲胎蛋白 (AFP)、白蛋白、波形蛋白等标志物的胎肝干细胞集落。证实人胎肝中同样存在具有干细胞特性的原始细胞。这一发现为肝干细胞的生物学特性的深入研究奠定了良好的基础
In order to investigate the existence of stem cells in human fetal liver and to identify their biological characteristics, primary isolation of human fetal hepatocyte colonies was used to detect the expression of cell colonies molecular markers by immunohistochemistry. Results: Fetal liver stem cell colonies expressing markers of AFP, albumin and vimentin were successfully isolated from human fetal liver tissue. Confirmed that human fetal liver also exists in the original cell characteristics of stem cells. This finding has laid a good foundation for the further study of the biological characteristics of liver stem cells