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目的 制备出小肠结肠炎耶尔森菌血清O∶3型特异的单克隆抗体。方法 用常规的骨髓杂交瘤融合技术。结果 筛选到了一株针对于小肠结肠炎耶尔森菌O∶3血清型特异性的克隆株 (编号 :Y6H8) ,且证实分泌的抗体属于针对于脂多糖免疫球蛋白IgG ,可用于玻片凝集法鉴定 0∶3血清型小肠结肠炎耶尔森菌。 结论 通过本实验证实小肠结肠炎耶尔森菌血清O∶3型菌株脂多糖存在其特异性成分 ,可用于该菌株的分型鉴定
Objective To prepare Yersinia enterocolitica serotype O: 3 specific monoclonal antibody. Methods Using conventional bone marrow hybridoma fusion techniques. As a result, a clone (Y6H8) specific to the Yersinia enterocolitica O: 3 serotype was screened and the secreted antibody was confirmed to belong to the IgG of the lipopolysaccharide immunoglobulin for use in slide agglutination Law 0: 3 serotype Yersinia enterocolitica. Conclusion The experiment confirmed that Yersinia enterocolitica serotype O: 3 lipopolysaccharide has its specific components and can be used to identify the strains