目的:研究黄连配伍吴茱萸后对盐酸小檗碱(ber)大鼠肝脏代谢以及对大鼠肝脏药物代谢酶活性的影响,探讨黄连吴茱萸药对配伍的机制。方法:HPLC测定大鼠肝微粒体温孵体系中ber的含量,采用在体诱导-体外肝微粒体温孵的方法,考察ber在吴茱萸、黄连、连萸配伍(6∶1)诱导组及溶媒对照组肝微粒体系中的代谢变化;采用Nash显色,分光光度法测定空白组、阳性药物组(酮康唑)、黄连组、吴茱萸组、连萸配伍(6∶1)组大鼠肝微粒体中红霉素N-脱甲基酶(ERD)、氨基比林N-脱甲基酶(ADM)活性。结果:与空白对照组相比,ber在各给药组大鼠肝微粒体温孵液中的代谢速率均有明显下降(P<0.001),且代谢速率连萸配伍组<吴茱萸组<黄连组<空白对照组。与空白组大鼠比较,酮康唑组、吴茱萸组和连萸配伍组ERD活性均明显受到抑制(P<0.01),同时吴茱萸和连萸配伍组还可使ADM活性显著降低(P<0.01);黄连组对ADM活性抑制作用明显(P<0.05),但对ERD活性作用不明显。结论:连萸配伍合用通过抑制大鼠肝药酶ERD和ADM活性降低黄连主要有效成分盐酸小檗碱的肝脏代谢,这可能是连萸配伍相互作用的重要机制。 Objective: To study the effects of Coptis chinensis Franch and Fructus Evodiae on hepatic metabolism of berberine hydrochloride (Ber) rats and on hepatic drug metabolizing enzyme activities in rats, and explore the mechanism of Evodia rutaecarpa compatibility. Methods: The content of berberine in rat liver microsomal incubation system was determined by HPLC. The in vitro induction of in vitro liver microsomal incubation method was used to study the effect of berberine on the induced group and the vehicle control group Hepatic microsomal system metabolic changes; using Nash colorimetry, spectrophotometric determination of blank group, the positive drug group (ketoconazole), Coptis, Evodia rutaecarpa group (6: 1) group liver microsomes Erythromycin N-demethylase (ERD), aminopyrine N-demethylase (ADM) activity. RESULTS: Compared with the blank control group, the metabolic rate of berberine in the liver microsomal incubation solution of each administration group was significantly decreased (P <0.001), and the metabolic rate of Fructus Corni combined group