Objective: To investigate the antihypertensive, antioxidant and renal protective effects of Eulcommia glycosides on renal hypertensive rats with two-kidney and one-clip type.Materials and Methods:A total of 50 healthy male SD rats were fed adaptive for one week, and after 2 weeks of 2K1C operation, they were divided into 5 groups: sham operation group, model control group, benazepri group, ulcommin high-dose group and low-dose group, with 8 rats in each group. Rats in each group were given continuously intragastric administration for 4 weeks, and systolic blood pressure of caudal artery was measured before modeling, 2 weeks after modeling, and after treatment. Urinary microalbumin (MALB) was measured at 24 hours. Serum AngⅡ and ROS were measured by taking blood from abdominal aorta. The right kidney of rats was taken to measure the expression level of NOX4mRNA and pathological observation by HE staining.Results: Compared with sham operation group, blood pressure, serum AngⅡ, ROS content, NOX4 mRNA expression level and MALB in kidney tissue of model group were significantly increased (P<0.05). Compared with model control group, the above indexes in treatment groups were significantly decreased (P<0.05). There was no statistical significance among all treatment groups (P<0.05). Under light microscope, normal glomeruli and renal tubules were observed in rats of sham operation group. Slight glomerular atrophy andfibrous hyperplasia were observed in the renal tissue of model control rats, and inflammatory cells were observed in renal interstitium. The above symptoms were slightly relieved in the treatment group compared with the model control group.Conclusion: Eucommia glycosides can reduce blood pressure and protect the kidney, and the mechanism may be related to the inhibition of renal oxidation.