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本文对传统藻胆蛋白的分离方法进行改进，利用柱层析法直接从新鲜多管藻提取分离出纯的Ｒ－藻蓝蛋白。分别用凝胶柱层析法和电泳法测定了其分子量。结果表明：通常实验条件下：Ｒ－藻蓝蛋白的最稳定聚集态是三聚体，其分子量为１２２．８ＫＤ，它由分子量分别为１８．１ＫＤ（α）和２０．５ＫＤ（β）两个亚基组成，其分子组成为（αβ）３。利用吸收和荧光光谱研究了Ｒ－藻蓝蛋白的光谱性质。Ｒ－ＰＣ的三聚体在可见光范围有两个明显的吸收峰，分别为５４６ｎｍ和６１４ｎｍ，与同系的Ｃ－ＰＣ和ＰＥＣ明显不同，表明各类色团光谱特性在三聚体状态基本不变，说明三聚体内色团间无强相互作用。Ｒ－ＰＣ荧光发射峰位于６４０ｎｍ，与同系的Ｃ－ＰＣ和ＰＥＣ基本一致，说明三聚体保证了高效的能量传递。单体的荧光发射呈现双峰（５６６ｎｍ，６４３ｎｍ），说明单体内能量传递效率不高。从光谱性质可知，在ＰＣ家族中，Ｒ－ＰＣ具有最高的光能捕获效率
In this paper, the separation of traditional phycobiliproteins was improved, and pure R-phycocyanin was extracted directly from fresh multi-tube algae by column chromatography. Their molecular weights were determined by gel column chromatography and electrophoresis respectively. Under normal experimental conditions, the most stable aggregation state of R-phycocyanin is a trimer with a molecular weight of 122.8KD. It consists of two molecular weights of 18.1KD (α) and 20.5KD (β) Subunit composition, the molecular composition of (αβ) 3. The spectroscopic properties of R-phycocyanin were studied by absorption and fluorescence spectroscopy. R-PC trimers have two distinct absorption peaks in the visible range of 546 nm and 614 nm, respectively, which are significantly different from the homologous C-PC and PEC, indicating that the spectral properties of the various chromophores are essentially unchanged in the trimer state , Indicating no strong interaction between the chromophore trimer. R-PC fluorescence emission peak at 640nm, with the same C-PC and PEC basically the same, indicating that the trimer to ensure efficient energy transfer. Fluorescence emission of the monomer showed a doublet (566nm, 643nm), indicating that the monomer energy transfer efficiency is not high. From the spectral properties, R-PC has the highest light capture efficiency in the PC family