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本研究采用外用血单核细胞,以SiO_2作为刺激因子,经培养后,Sephadex G—75凝胶柱层析分离IL—1,观察IL—1对牙龈成纤维细胞PGE_2的产生和细胞增殖的影响,PGE_2的测定采用放免分析,以~3H—TdR掺入反映增殖情况。发现IL—1明显促进牙龈成纤维细胞PGE_2的产生,也明显促进牙龈成纤维细胞增殖,说明IL—1能通过促进PGE_2的产生而介导一系列牙周组织的病理过程,另外,还能通过促进牙龈成纤维细胞的增殖而在牙周组织修复中而起着重要作用。
In the present study, peripheral blood mononuclear cells (PBMCs) were treated with SiO_2 as stimulating factor. IL-1 was separated by Sephadex G-75 gel filtration column chromatography. The effect of IL-1 on PGE_2 production and cell proliferation in gingival fibroblasts , PGE_2 determination by radioimmunoassay, ~ 3H-TdR incorporation reflect the proliferation. IL-1 was found to significantly promote the production of PGE 2 in gingival fibroblasts and also promote the proliferation of gingival fibroblasts. IL-1 can mediate the pathological process of a series of periodontal tissues by promoting the production of PGE 2. In addition, Promote the proliferation of gingival fibroblasts in periodontal tissue repair and plays an important role.