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The GC analysis of fatty acid of four species of marine microalgae cultured at different temperature shows that the temperature has distinct effects on the composition and unsaturated degree of fatty acid of microalgae. The percentages of TPUFA in TFA and the number of mean double-bond of fatty acids ot Isochrysis galbana, Dunaliella salina and Phaeodactylum tricornutum decrease with the ascending temperature, while those of TMUFA and TSFA increase under the conditions of the present experiment. The content of TPUFA and the number of mean double-bonds of Chlorella sp. first decrease and then increase, with the minimum occurring at 20℃.