论文部分内容阅读
分别取皱纹盘鲍 (Haliotisdiscushannai)足肌和血液制样 ,经DAPI染色后 ,用PartecPAS -Ⅲ型流式细胞仪测定其倍性 ,试验证明三倍体鲍的DNA相对含量是二倍体的 1 5倍 ,切足法和抽取血液法两种制样方法均能得到满意结果 ,用流式细胞仪法检测鲍倍性适应鲍多倍体产业化的需要
Haliotis discovana hamster muscle and blood samples were prepared respectively. After DAPI staining, the ploidy of Parthenocissus dorsalis was determined by PartecPAS-III flow cytometry. The results showed that the relative content of DNA in diploid abalone was diploid 1 5 times, cut foot method and blood sampling two sample preparation methods can get satisfactory results, using flow cytometry to detect abalone to adapt abalone industrialization needs