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目的 研究白眉蝮蛇 (Agkistrodonhalysussuriensis)毒中精氨酸酯酶的致突变作用。 方法 用鼠伤寒沙门细菌营养缺陷型突变株TA97,TA98,TA10 0和TA10 2 ,采用平皿掺入法进行Ames试验 ,将实验分为加和不加代谢激活系统S92组平行试验。精氨酸酯酶设 6个浓度 :10 .0× 10 -3 ,5 .0× 10 -3 ,2 .5× 10 -3 ,1.2 5× 10 -3 ,0 .6 2 5× 10 -3 和 0 .312× 10 -3 U/mL。结果 加和不加代谢激活系统S9两种条件下 ,精氨酸酯酶不诱发鼠伤寒沙门细菌营养缺陷型突株的回复突变。Ames试验结果为阴性。结论 从致突变角度考虑 ,精氨酸酯酶在高于“蝮蛇清栓酶”(主要成分为精氨酸酯酶 )临床治疗剂量约 10 0 0倍的条件下仍然较安全。
Objective To study the mutagenesis of arginase in Agkistrodon halysussuriensis. Methods Salmonella typhimurium Salmonella typhimurium mutants TA97, TA98, TA10 0 and TA10 2 were treated with Ames test by plate incorporation method. The experiment was divided into two groups with and without SAC. Arginine esterase was set at six concentrations: 10 .0 × 10 -3, 5.0 × 10 -3, 2.5 × 10 -3, 1.2 5 × 10 -3, 0.626 × 10 -3 And 0 .312 × 10 -3 U / mL. Results Both arginine esterase did not induce the back mutation of Salmonella typhimurium auxotrophic mutant strain under the two conditions of addition and non-metabolism-activated system S9. Ames test result is negative. Conclusion From the point of view of mutagenesis, arginine esterase is still safer than about 100 000 times the clinical therapeutic dose of “Huoqingqingxinase” (the main component is arginine esterase).