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目的明确流脑菌群在濮阳市的分布及W135群在不同人群中的基因型别,为防控流脑提供依据。方法对健康人群、密切接触者的咽拭子,流脑患者的脑脊液、血液进行细菌培养、血清凝集、PCR鉴定、MLST基因分型、Por A基因亚型分型。结果 2009年-2013年健康人群检出Nm菌49株(B群27株,C群3株,W135群5株,未定群14株);流脑患者W135群1株;密切接触者W135群2株。流脑患者与密切接触者W135群菌株MLST基因分型均为ST-11,Por A VR基因亚型均为P1.5,2型。结论濮阳市流脑带菌以B群、W135群、C群为主,带菌谱与流脑发病有一定关联,有高致病性的W135群ST-11克隆系和致病性菌株P1.5,2型存在。应注意流脑菌群变化,加大监测和疫苗的应用,预防流脑流行。
Objective To clarify the distribution of meningococcal flora in Puyang and the genotypes of W135 in different populations to provide the basis for prevention and control of meningitis. Methods Bacterial culture, serum agglutination, PCR identification, MLST genotyping and Por A genotyping were performed on throat swabs and cerebrospinal fluid of patients with cerebrovascular disease. Results In 2009-2013, 49 strains of Nm were detected in healthy population (27 strains of B group, 3 strains of C group, 5 strains of W135 group and 14 strains of undetermined group), 1 W135 group of meningitis group, 1 case of W135 group 2 Strain. The genotypes of MLST genotypes of W135 strains were closely related to ST-11 and Por A VR genotypes were P1.5 and 2, respectively. Conclusion The prevalence of meningococcal meningitis in Puyang City is mainly related to B group, W135 group and C group, and the carrier spectrum is associated with the pathogenesis of meningitis. The highly pathogenic W135 group ST-11 clone and pathogenic strain P1.5, Type 2 exists. Should pay attention to changes in meningococcal flora, increase the monitoring and vaccine application, prevent meningococcal epidemic.