以完熟水果为材料提取RNA，除了要最大限度地抑制内源RNA酶活性，严格杜绝外源RNA酶污染外，选择合适的试验材料也是试验成功的关键。选取健壮植株上发育正常的果实，剖取果皮或近果皮果肉组织，在Chomczynski硫氰酸肌一步法的基础上，进行了2次变性，反复抽提，无菌操作等技术改进，获得了满意的纯化效果。 In order to extract the RNA from the ripe fruit, we must select the suitable test material to test the key to the success of the experiment except to minimize the endogenous RNase activity and strictly eliminate the exogenous RNase contamination. Select the healthy plants on the development of normal fruit, cut the pericarp or pericarp pulp tissue, on the basis of Chomczynski thiocyanate muscle one-step method, the two denaturation, repeated extraction, aseptic technique and other technical improvements, obtained satisfaction Purification effect.