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比较了热水回流法、热水-酶回流法、超声波法以及超声波-酶水解法等4种提取方法对灵芝菌丝体中β-葡聚糖提取得率的影响。结果表明,采用超声波-酶水解法所得β-葡聚糖的得率最大,影响提取的关键因素为超声波功率(A),水料比(B),p H(C)和加酶量(D)。进一步采用Box-Behnken设计及响应面分析法对这4个因素进行了优化。结果显示,因素A、B、C对β-葡聚糖提取得率的影响达到极显著水平(P<0.01),但因素D和所有因素之间的交互作用并不显著(P>0.05)。通过回归拟合,建立了预测灵芝β-葡聚糖提取的多项式模型。经响应面最优分析,获得4个因素的最佳水平为:超声波功率275.54W,水料比25.62:1(V/M,m L/g),p H 7.17,加酶量1.05%。经实际试验验证,在该条件下,灵芝菌丝体β-葡聚糖得率可达5.50mg/g。
The effects of hot water reflux, hot water-enzyme reflux, ultrasonic method and ultrasonic-enzymatic hydrolysis on the yield of β-glucan from Ganoderma lucidum mycelium were compared. The results showed that the yield of β-glucan obtained by ultrasonic-enzymatic hydrolysis was the highest, and the key factors influencing the extraction were ultrasonic power (A), water and material ratio (B), p H (C) and enzyme dosage ). These four factors were further optimized using Box-Behnken design and response surface methodology. The results showed that the effects of A, B and C on the yield of β-glucan were significant (P <0.01), but the interaction between factor D and all the factors was not significant (P> 0.05). By regression fitting, a polynomial model was established to predict the β-glucan extraction of Ganoderma lucidum. According to the optimal response surface analysis, the best four factors were obtained: ultrasonic power 275.54W, water / material ratio 25.62: 1 (V / M, m L / g), pH 7.17, enzyme dosage 1.05%. The experimental results show that under this condition, the yield of β-glucan of Ganoderma lucidum mycelium can reach 5.50mg / g.