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目的建立交通相关的PM2.5对大鼠亚急性染毒模型,观察其对大鼠血清及肺泡灌洗液中细胞因子水平的影响,进一步探讨其免疫毒性。方法将24只体重为180～200 g的雄性SD大鼠随机分为4组,即生理盐水对照组、PM2.5低、中、高剂量组(1.5、6和24 mg/kg)。采用隔天气管滴注染毒颗粒物,共6次,最后一次染毒后24 h内处死大鼠。收集血清和肺泡灌洗液,用ELISA法测定细胞因子白介素-2(IL-2)、白介素-4(IL-4)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果 PM2.5染毒组大鼠血清和肺泡灌洗液中IL-2、IL-4水平随染毒剂量的增加逐渐降低,中、高剂量组与生理盐水对照组相比,差异均有统计学意义(P<0.05)。与生理盐水组比较,各染毒组大鼠血清IL-6和TNF-α水平升高,除低剂量组外,其余差异均有统计学意义(P<0.05)。结论交通相关的PM2.5可引起大鼠炎症细胞因子水平的改变,此反应可能与其引起的免疫毒性反应密切相关。
OBJECTIVE: To establish a subacute model of transport-related PM2.5 in rats and observe the effect of PM2.5 on the levels of cytokines in serum and BALF in rats, and further investigate its immunotoxicity. Methods Twenty-four male Sprague-Dawley rats weighing 180-200 g were randomly divided into 4 groups: normal saline control group, PM2.5 low, medium and high dose groups (1.5, 6 and 24 mg / kg). Adopted intravenous drip every other day for 6 times, and the rats were sacrificed within 24 hours after the last exposure. Serum and alveolar lavage fluid were collected and the levels of IL-2, IL-4, IL-6, TNF-α )Level. Results The levels of IL-2 and IL-4 in the serum and BALF of PM2.5-treated rats decreased gradually with the increase of the dose, and there were statistical differences between the medium and high dose groups and the saline control group Significance (P <0.05). Compared with the saline group, the levels of IL-6 and TNF-α in the serum of rats in each exposure group increased, except for the low dose group, the difference was statistically significant (P <0.05). Conclusion Traffic-related PM2.5 can cause changes in inflammatory cytokines in rats, which may be closely related to the immunotoxic reactions.