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内皮细胞培养是研究内皮细胞生物学的重要方法。近年国外开展内皮细胞长期培养,并用于细胞生物学,动脉粥样硬化病理学、血栓形成等机制的研究中。本文在国内首次对长期培养的人血管内皮细胞进行超微结构的研究。本实验按[1]的方法进行脐蒂静脉内皮细胞的长期培养达25代(积累倍增数60-75,倍增时间20-25小时)。按[1、2]方法对流产胎儿主动脉内皮细胞长期培养达10-15代、(积累倍增数25-30,倍增时间20-25小时)。
Endothelial cell culture is an important method of studying endothelial cell biology. In recent years, foreign long-term endothelial cell culture, and for cell biology, atherosclerosis pathology, thrombosis mechanisms such as research. This article for the first time in the long-term cultured human vascular endothelial cells ultrastructural studies. The experiment according to [1] method of umbilical venous endothelial cells cultured for up to 25 generations (fold doubling 60-75, doubling time 20-25 hours). According to the [1,2] method of abortion fetus aortic endothelial cells for a long-term culture of up to 10-15 generations (double fold accumulation of 25-30, doubling time 20-25 hours).