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小鼠骨髓细胞在体外培养中,加入用流式自由电泳法分离所得的高纯度正常B淋巴细胞,可使多向祖细胞(CFU-mix)集落增加至5倍;加入小鼠B淋巴瘤细胞株的条件培基(M_(12.4.1)-CM)时,CFU-mix数也可增加至4倍。单集落形态学分析结果表明M_(12.4.1)-CM可加强CFU-GEMm及p-BFU-E等早期造血祖细胞的增殖与分化。小鼠高纯度B细胞样品在体外培养中加入1000 rad照射的骨髓细胞可出现CFU-mix集落,如果再加入适量的小鼠肺条件培基,则CFU-mix数量比对照大15倍,其集落性质为CFU-GEMm,GMm及p-BFU-E。在此培养中加不同稀释度抗小鼠IgM血清,结果CFU-mix的产率与抗IgM血清的浓度成直线反比关系,当加入1:10抗小鼠IgM血清时,CFU-mix为0。作者假设在一定培养条件下,IgM阳性的部分B细胞可返祖转化为CFU-mix。
Mouse bone marrow cells were cultured in vitro, by adding the flow-free electrophoresis separation of the resulting high-purity normal B lymphocytes, multipotential progenitor cells (CFU-mix) colonies increased to 5 times; adding mouse B lymphoma cells Strain conditioned medium (M_ (12.4.1) -CM), CFU-mix number can also be increased to 4 times. Morphological analysis of single colonies showed that M (12.4.1) -CM enhanced the proliferation and differentiation of early hematopoietic progenitor cells such as CFU-GEMm and p-BFU-E. CFU-mix colonies appeared in 1000-rad irradiated bone marrow cells of mouse high-purity B-cell samples, and CFU-mix was 15 times larger than that of the control if an additional amount of mouse lung conditioned medium was added. Colonies The properties of CFU-GEMm, GMm and p-BFU-E. Different dilutions of anti-mouse IgM sera were added to this culture. As a result, the yield of CFU-mix was inversely proportional to the concentration of anti-IgM serum and CFU-mix was 0 when 1: 10 anti-mouse IgM serum was added. The authors hypothesized that some cultures of IgM-positive B cells could be converted to CFU-mix under certain culture conditions.