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目的观察溪黄草水提物对α-萘异硫氰酸酯(ANIT)诱发的肝内胆汁淤积大鼠的影响。方法采用ANIT 80 mg·kg-1复制大鼠肝内胆汁淤积模型,健康SD雄性大鼠按体质量随机分为6组,每组8只,即:空白对照组,溪黄草高剂量组(60 g·kg-1),溪黄草中剂量组(40 g·kg-1),溪黄草低剂量组(20 g·kg-1),模型组和熊去氧胆酸(UCUD)组(100 mg·kg-1)。灌胃给药后,进行胆总管插管手术,观察溪黄草对大鼠1.5 h内的胆汁流量以及血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、γ-谷氨酰转移酶(γ-GT)、总胆固醇(TC)、直接胆红素(DBIL)、总胆红素(TBIL)、总胆汁酸(TBA)的影响。结果与模型组比较,溪黄草各剂量组各项指标(除ALT、ALP、γ-GT外)均有一定改善(P<0.05,P<0.01),但部分未呈现一定量效关系。UCUD组对AST有改善,其他7项指标效果不明显。与模型组比较,溪黄草中、高剂量组以及UCUD组胆汁流量均有显著提高(P<0.05,P<0.01)。病理切片结果显示溪黄草高剂量组和UCUD组对ANIT诱发的肝细胞损伤有所改善。结论溪黄草对ANIT诱发的实验性大鼠肝内胆汁淤积损伤具有一定干预改善作用。
Objective To observe the effects of aqueous extract from Xihuang Cao on intrahepatic cholestasis induced by α-naphthalene isothiocyanate (ANIT). Methods Rat model of intrahepatic cholestasis was established by ANIT 80 mg · kg-1. The healthy SD male rats were randomly divided into 6 groups according to their body weight, with 8 rats in each group: blank control group, high dose of Xihuangcao group 60 g · kg-1, 40 g · kg-1 of Xihuangcao low dose group, 20 g · kg-1 of Xihuangcao group, 100 of model group and UCUD group mg · kg-1). After gavage administration, the bile duct intubation was performed to observe the effects of Xihuangcao on the bile flow within 1.5 h and the changes of serum ALT, ALT, ALP, (Γ-GT), total cholesterol (TC), direct bilirubin (DBIL), total bilirubin (TBIL) and total bile acid (TBA) Results Compared with the model group, the indexes in all dose groups (all except ALT, ALP and γ-GT) were improved (P <0.05, P <0.01), but some did not show a dose-response relationship. UCUD group improved AST, the other seven indicators of the effect is not obvious. Compared with the model group, the biliquid fluxes of middle and high dose of Xihuangcao and UCUD group were significantly increased (P <0.05, P <0.01). The results of pathological examination showed that Xihuangcao high-dose group and UCUD group improved the ANIT-induced hepatocyte injury. Conclusion Xihuangcao can improve the intrahepatic cholestasis induced by ANIT in rats.