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EA_(36)在巴氏染色中配制复杂,药品昂贵,使用短暂。本文用一种简便、快速、节俭的配制方法延长了染液的使用时间。0.5%淡绿液45ml,0.5%俾士麦褐液10ml,0.5%伊红液45ml,混合搅匀。将磷钨酸0.5—1.0g%徐徐加入混合液中,边加边搅,同时不断滴一滴到滤纸上,直到出现一分界清晰的同心圆排列的三色彩图(中心鲜绿色,中环棕褐色,外环黄红色)为止。EA_(36)使用过久后,胞浆均染成棕灰色,此时取染液一滴于滤纸上,三色形环图消失。遇此情况时,在原染液中重新加入磷钨酸,直至滤纸上重新出现三色彩环图,即可继续使用。
EA_ (36) Complex preparation in pasteurization, expensive drugs, use short. This article uses a simple, fast, thrifty preparation method to extend the use of bath time. 0.5% light green liquid 45ml, 0.5% Bismarck brown liquid 10ml, 0.5% Yili liquid 45ml, mix well. Phosphotungstic acid 0.5-1.0g% slowly added to the mixture, while adding while stirring, while dropping a drop of filter paper until a clearly defined concentric circle arrangement of the three color picture (center of the bright green, the central brown, Outer ring yellow-red) so far. EA_ (36) used too long, the cytoplasm were stained brown, then took a drop of dye on the filter paper, tricolor ring diagram disappeared. In this case, re-add phosphotungstic acid in the original dye until the filter paper re-appearance of three-color ring diagram, you can continue to use.