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为研究大鼠副交感神经对妊娠早期子宫内组织胺(HA)和血清白血病抑制因子(LIF)的影响,选取180~200日龄健康性成熟Wistar雌性大鼠20只,随机分为对照组和处理组,处理组采用腹腔注射阿托品阻断副交感神经,对照组腹腔注射0.9%生理盐水。结果表明:阿托品处理后妊娠5d子宫囊胚数量明显减少、囊胚发育延迟;妊娠6d虽然囊胚数量接近正常对照组数量,但胚胎凸起仍小于正常妊娠6d状态。子宫肥大细胞(MC)释放HA结果显示,阻断副交感神经激发MC释放的HA量显著高于正常状态,且与MC自发释放的HA变化趋势不同;正常对照组血清LIF含量的变化趋势是随着妊娠时间的增加而减少,而处理组出现相反的趋势。试验结果提示,在着床窗口期副交感神经通过对MC释放HA等介质调控子宫的生理功能,HA和LIF分泌规律紊乱对胚胎着床的影响更为显著,导致囊胚发育延迟,甚至丢失。
In order to study the effect of parasympathetic nerve on uterine histamine (HA) and serum leukemia inhibitory factor (LIF) in early pregnancy, 20 healthy adult Wistar female rats aged 180 ~ 200 days were randomly divided into control group and control group Group, the treatment group by intraperitoneal injection of atropine block parasympathetic nerve, the control group by intraperitoneal injection of 0.9% saline. The results showed that the number of uterine blastocysts was significantly decreased and the development of blastocyst was delayed on the 5th day of gestation after atropine treatment. Although the number of blastocysts was close to that of the normal control group on the 6th day of gestation, the embryo protrusion was still smaller than the 6th day of normal pregnancy. The release of HA by uterine mast cells (MCs) showed that the amount of HA released from MCs blocked by parasympathetic nerve activation was significantly higher than that of normal condition, and the trend of HA release from spontaneous release of MC was different. The trend of serum LIF content in normal control group was The increase of pregnancy time decreased, while the treatment group appeared the opposite trend. The test results suggest that the parasympathetic nerve affects the uterine function by regulating the release of HA and other mediators in the implantation window. The disorder of HA and LIF secretion has a more significant effect on embryo implantation, resulting in delayed or even lost blastocyst development.