论文部分内容阅读
背景:神经生长因子(nervegrowthfactor,NGF)不仅是维持促进中枢神经系统发育、分化、存活的基本的生长因子,而且在外周神经损伤的修复中也起着重要的作用。目的:观察NGF肌注对大鼠坐骨神经挤压伤后神经再生恢复以及功能的影响。设计:以实验动物为研究对象,随机对照的重复观察测量,探索性研究。单位:一所军医大学的新药评价中心。材料:实验于1999-07/2000-03在第二军医大学基础部新药评价中心完成。SD大鼠40只,雌雄各半,体质量200～250g,上海西普尔-必凯实验动物有限公司提供。方法:将40只大鼠随机分成NGF高、中、低剂量组,正常对照和模型对照组。距坐骨切迹远端6mm处钳夹坐骨神经,使产生一4mm宽的挤压伤。NGF高、中、低剂量组分别给予NGF8,4和2μg/kg(1.6×103,8×102和4×102IU/kg)药物,肌注1次/d,连续56d。主要观察指标:①手术后的不同时间神经传导速度(nerveconductionve-locities,NCV)和坐骨神经功能指数(sciaticfunctionalindex,SFI)。②组织形态学评价。结果:与模型对照组相比,高剂量组在35,56d,中剂量组在35d的NCV均显著加快(t=2.32～5.14,P<0.05～0.01);高、中、低3个剂量组在手术14d后的SFI与模型组相比,差异均有显著性意义(t=2.29～6.28,P<0.05～0.01),且高剂量组恢复较明显,至56d各剂量组SFI
BACKGROUND: Nerve growth factor (NGF) is not only a basic growth factor that maintains the development, differentiation and survival of the central nervous system, but also plays an important role in the repair of peripheral nerve injury. OBJECTIVE: To observe the effects of NGF intramuscular injection on the recovery and function of nerve regeneration after sciatic nerve crush injury in rats. Design: The experimental animals as the research object, randomized controlled repeated observation measurement, exploratory research. Unit: a new drug evaluation center of Military Medical University. MATERIALS: The experiment was performed at the Evaluation Center for New Drugs of the Second Military Medical University from July 1999 to March 2000. 40 SD rats, male and female, body weight 200 ~ 250g, Shanghai Xi Pur - will Kay Experimental Animal Co., Ltd. to provide. Methods: Forty rats were randomly divided into NGF high, medium and low dose groups, normal control group and model control group. A distance of 6 mm from the distal end of the sciatic notch impacted the sciatic nerve, resulting in a 4 mm wide crush injury. NGF high, medium and low dose groups were given NGF8, 4 and 2μg / kg (1.6 × 103, 8 × 102 and 4 × 102IU / kg) drugs, intramuscular injection 1 time / d, continuous 56d. MAIN OUTCOME MEASURES: ① nerve conduction velocity (NCV) and sciatic functional index (SFI) at different time points after operation. ② histological evaluation. Results: Compared with the model control group, the NCV of the high dose group was significantly increased at 35 and 56 days (P <0.05) and that of the middle dose group at 35 days (t = 2.32-5.14, P <0.05-0.01) Compared with the model group, the difference of SFI in the 14th day after operation was significant (t = 2.29 ~ 6.28, P <0.05 ~ 0.01), and the recovery in the high dose group was more obvious. The SFI