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目的：观察大鼠长期口服黄苓茎叶总黄酮（ＴＦ）产生的毒性反应及其严重程度，毒性反应的耙器官及其损害的可逆性，为确定人用安全剂量提供依据。方法：Ｗｉｓｔａｒ大鼠，分为０．５、１．０、２．０ｇ·ｋｇ－１ＴＦ和ＣＭＣ对照组，灌胃给药，连续９０ｄ，观察用ＴＦ９０ｄ和停药１４ｄ时对大鼠血液学、血液生化学、重要脏器系数及病理组织学的影响。结果：用药９０ｄ时２．０ｇ·ｋｇ－１ＴＦ组大鼠ＲＢＣ高于ＣＭＣ对照组，停药１４ｄ恢复正常。其它组大鼠的各项指标均无异常改变，也未发现迟发性毒性反应。结论：本实验所用高剂量高达小鼠最大耐受量的１／５，除出现一过性胃肠道毒性反应及用药９０ｄ时ＲＢＣ升高外，未见明显毒性反应，提示ＴＦ毒性较低
OBJECTIVE: To observe the toxicity and severity of long-term oral administration of total flavonoids (TFs) in Huangling and its reversibility in harming organs and toxicity, and provide the basis for the determination of safe dosage for human use. Methods: Wistar rats were divided into 0.5, 1.0, 2.0g · kg-1TF and CMC control groups. The rats were administered intragastrically for 90 days. The effects of TF90d and withdrawal for 14 days on the hematology, Blood biochemistry, vital organ coefficient and histopathology. Results: The RBC of 2.0g · kg-1 TF group was higher than that of CMC control group at 90 days after drug administration, and returned to normal after 14 days of drug withdrawal. Other groups of rats showed no abnormal changes in the indicators, nor delayed toxic reactions. Conclusion: In this experiment, the high dose up to 1/5 of the maximum tolerable dose of mice, in addition to a transient gastrointestinal toxicity and RBC increased 90 days after treatment, no significant toxicity, suggesting that TF toxicity is low