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应用傅里叶变换红外光谱(FTIR)联合主成分分析法(PCA),分析直肠癌转移淋巴结的谱学特征,并对直肠癌转移淋巴结和非转移淋巴结进行线性判别分析。80例直肠癌转移淋巴结和80例未转移淋巴结进行FTIR光谱分析,计算峰强并进行主成分分析,得出在波数4 000~1 700cm~(-1)范围主成分1(Principal components 1,PC1)是3 260cm~(-1),PC2为1 740cm~(-1)。波数1 700~1 000cm~(-1)范围,PC1为1 640cm~(-1),PC2为1 080cm~(-1),将良、恶性淋巴结光谱3 260,1 740,1 640,1 080cm~(-1)相对峰强比(I/I1 460)和波数1 080和1 300cm~(-1)进行t检验,良、恶性结果差异有统计学意义(p<0.05),表明癌转移淋巴结中蛋白含量、蛋白的形成、氨基酸增多;脂肪含量明显减少与癌组织中无氧酵解脂肪含量减少有关。将相对峰强比(I1 080/I1 460,I1 640/I1 460,I3 260/I1 460,I1 740/I1 460,n=160)进行PCA聚类分析,结果显示可以将良恶性淋巴结鉴别,良性淋巴结聚类在第一和四象限,恶性淋巴结聚类在二和三象限。将相对峰强比、1 080和1 300cm~(-1)进行线性判别分析(LDA),将25例淋巴结作为验证集进行分析,得出PCA/LDA模型的敏感度是87.5%,特异度是88.5%。结果表明傅里叶变换红外光谱分析技术可成为术中原位、在体和快速诊断直肠癌淋巴结转移的一种简便方法。
Fourier transform infrared spectroscopy (FTIR) combined with principal component analysis (PCA) was used to analyze the spectral features of metastatic lymph nodes in rectal cancer. Linear discriminant analysis was performed on metastatic lymph nodes and non-metastatic lymph nodes. 80 cases of rectal cancer metastasis lymph nodes and 80 cases of non-metastatic lymph nodes were analyzed by FTIR spectroscopy to calculate the peak intensity and the principal component analysis, obtained in the wave number 4 000 ~ 1 700cm -1 Principal Component 1 (PC1 ) Is 3 260 cm -1, PC 2 is 1 740 cm -1. The wavenumbers ranged from 1 700 to 1 000 cm -1, PC 1 was 1 640 cm -1, PC 2 was 1 080 cm -1, and the benign and malignant lymph node spectra were 3 260,1 740,1 640 and 1 080 cm (-1) relative peak intensity ratio (I / I1 460) and wavenumber 1 080 and 1 300 cm -1, the difference between benign and malignant results was statistically significant (p <0.05), indicating that the metastatic lymph node Protein content, protein formation, amino acids increased; fat content decreased significantly with the reduction of anaerobic glycolysis in cancer tissue fat content. PCA clustering analysis of relative peak to peak ratio (I1 080 / I1 460, I1 640 / I1 460, I3 260 / I1 460, I1 740 / I1 460, n = 160) showed that benign and malignant lymph nodes can be differentiated, benign Lymph node clustering In the first and fourth quadrants, malignant lymph nodes cluster in the second and third quadrants. Linear discriminant analysis (LDA) was performed on the relative peak to peak ratio, 1 080 and 1 300 cm -1, and 25 lymph nodes were used as the validation set. The sensitivity of the PCA / LDA model was 87.5% and the specificity was 88.5%. The results show that Fourier transform infrared spectroscopy technique can become a convenient method for in situ and in vivo and rapid diagnosis of lymph node metastasis in rectal cancer.