磷酸丙糖异构酶(TPI)被认为是曼氏血吸虫病一个很有希望的疫苗候选抗原。尚未见日本血吸虫TPI的报道,作者从日本血吸虫菲律宾株(SjP)和中国大陆株(SjC)获取了TPI全长克隆,本文着重报告其核苷酸序列及推算的氨基酸序列,以及血吸虫TPI基因的种间和株间差异。 按已发表的曼氏血吸虫TPI核苷酸序列设计一对引物,以SjP和SjC成虫基因组DNA为模板作PCR,扩增产物经克隆和双 Triosephosphate isomerase (TPI) is considered as a promising vaccine candidate for Schistosoma mansoni. Although the TPI of Schistosoma japonicum has not been reported, the authors obtained TPI full-length clones from S. japonicum SjP and SjC. This paper reports the nucleotide sequence and deduced amino acid sequence of TPI, Differences between species and strains. A pair of primers was designed according to the published nucleotide sequence of TPI of Schistosoma mansoni. PCR was carried out using the genomic DNA of SjP and SjC as template. The amplified product was cloned and double