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目的观察不同浓度川芎嗪干预后乳腺癌细胞侵袭、黏附能力的改变及MMP-2/MMP-9的表达。方法用细胞黏附实验,检测川芎嗪对MDA-MB-231细胞黏附力的抑制作用;用Transwell小室检测不同浓度川芎嗪干预后MDA-MB-231细胞体外侵袭能力的改变;用半定量逆转录-聚合酶联反应(RT-PCR)检测川芎嗪对MDA-MB-231细胞MMP-2/MMP-9 mRNA表达的抑制作用。结果川芎嗪对MDA-MB-231细胞黏附力有明显抑制作用,对MDA-MB-231细胞的MMP-2 mRNA的表达有明显抑制作用;但对MMP-9mRNA的表达无明显影响。结论川芎嗪能显著抑制MDA-MB-231细胞的黏附和侵袭能力,其抑制效应与药物的浓度呈正相关。川芎嗪可能通过下调MDA-MB-231细胞MMP-2mRNA的表达而抑制细胞的黏附、侵袭能力。
Objective To observe the changes of invasion, adhesion and the expression of MMP-2 / MMP-9 in breast cancer cells treated with different concentrations of ligustrazine. Methods Cell adhesion assay was used to detect the inhibitory effect of tetramethylpyrazine on the adhesion of MDA-MB-231 cells. Transwell chamber was used to detect the in vitro invasive ability of MDA-MB-231 cells after intervention with different concentration of ligustrazine. Semiquantitative reverse transcription- Polymerase chain reaction (RT-PCR) was used to detect the inhibitory effect of ligustrazine on the expression of MMP-2 / MMP-9 mRNA in MDA-MB-231 cells. Results Tetramethylpyrazine significantly inhibited the adhesion of MDA-MB-231 cells and inhibited the expression of MMP-2 mRNA in MDA-MB-231 cells. However, it had no effect on the expression of MMP-9 mRNA. Conclusion Tetramethylpyrazine can significantly inhibit the adhesion and invasion of MDA-MB-231 cells, and the inhibitory effect is positively correlated with the drug concentration. Tetramethylpyrazine may inhibit cell adhesion and invasion by down-regulating MMP-2 mRNA expression in MDA-MB-231 cells.