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目的和方法 :用细胞外记录法和多管微电极微电泳法在 36只麻醉自主呼吸的SD大鼠 ,系统探查旁巨细胞外侧核尾半侧 (cPGCL)呼吸神经元 (RNs)的类型及该区的递质受体情况。结果 :在 14只大鼠系统探查 ,记录到 39个RNs ,包括吸气神经元 2 4个、呼气神经元 12个和跨时相神经元 3个。在另 2 2只大鼠 ,观察到微电泳谷氨酸钠 (L Glu)使 12 / 14个RNs兴奋 ,γ 氨基丁酸 (GABA)使全部受试RNs抑制 (n =2 2 ) ;对NMDA受体拮抗剂DL 2 氨基 5 磷酸戊酸 (AP5)、GABAA 受体拮抗剂荷包牡丹碱 (BIC)有兴奋、抑制和无作用三类效应 ;AP5部分阻断大部分受试神经元 (6 / 9个 )对L Glu的兴奋反应 ,BIC部分或完全阻断大部分受试神经元 (9/ 11个 )对GABA的抑制反应。结论 :PGCL是呼吸调控的重要中枢部位之一 ,该区可能存在起神经递质作用的内源性L Glu和GABA及兴奋性氨基酸 (包括NMDA和非NMDA)受体和GABAA 受体 ,它们可能介导PGCL对呼吸的调控
OBJECTIVE AND TEST METHODS: Thirty-six Sprague-Dawley (SD) rats were anesthetized by extracellular recording and multi-tube microelectrode electrophoresis. The types of respiratory neurons (RNs) in the caudal lateral plexus (cPGCL) The area of the transmitter of the receptor situation. Results: Fourteen rats were sequenced and 39 RNs were recorded. There were 24 inspiratory neurons, 12 exhaled neurons and 3 neurons in interphase. Of the other 22 rats, 12/14 RNs were found to be excited by micro-electrophoresis of glutamate (GABA) and all of the tested RNs were inhibited (n = 2 2); NMDA AP5 partly antagonized, inhibited and no effect of GABAA receptor antagonist bicuculline (BIC); AP5 partially blocked most of the tested neurons (6 / 9) on L Glu, BIC partially or completely blocked the inhibitory response to GABA in most of the tested neurons (9 out of 11). CONCLUSION: PGCL is one of the important central respiratory control sites. There may be endogenous L Glu and GABA and excitatory amino acids (including NMDA and non-NMDA) receptors and GABAA receptors that may play the role of neurotransmitter in this area. Mediate PGCL regulation of respiration