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目的:分析人巨细胞病毒(HCMV)AD169株UL115基因序列,预测UL115基因编码的gL蛋白B细胞优势表位。方法:基于UL115基因编码的gL蛋白的氨基酸序列,结合亲水性参数、可及性参数、抗原性参数、柔韧性参数及二级结构方案对HCMVgL蛋白的B细胞表位进行预测,参照已建立的预测方法综合评价B细胞优势表位。结果:①UL115核酸变异集中在序列的N端,大部分是同义突变,氨基酸序列高度保守;②HC-MVgL蛋白B细胞表位可能位于编码蛋白N段197～205、253～261位。结论:多参数预测gL蛋白的B细胞优势表位,为进一步研究蛋白特征、制备单克隆抗体及表位疫苗提供依据。
OBJECTIVE: To analyze the sequence of UL115 gene of human cytomegalovirus (HCMV) AD169 strain and predict the dominant epitopes of gL protein B cells encoded by UL115 gene. Methods: The B cell epitope of HCMVgL protein was predicted based on the amino acid sequence of gL protein encoded by UL115 gene combined with hydrophilicity parameter, accessibility parameter, antigenicity parameter, flexibility parameter and secondary structure scheme. The prediction method of comprehensive evaluation of B cell dominant epitopes. Results: ①UL115 mutation concentrated on the N terminus of the sequence, mostly synonymous mutations, amino acid sequence highly conserved; ②HC-MVgL protein B cell epitopes may be located in the N-encoding protein 197 ~ 205,253 ~ 261. Conclusion: The multi-parameter prediction of the dominant epitopes of g-protein B lymphocytes provides a basis for further study of protein characteristics, preparation of monoclonal antibodies and epitope vaccines.