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目的：为了找到脐血中粒细胞集落刺激因子（Ｇ－ＣＳＦ）产生的部位，为脐血的基础研究和临床应用提供科学依据。方法：应用Ｇ－ＣＳＦＥＬＩＳＡ试剂盒采用酶联免疫方法，分别测定了４８例妊娠３７～３８周健康孕妇静脉血清和５０例正常足月新生儿脐静脉血清和４９例脐动脉血清Ｇ－ＣＳＦ阳性率和含量。结果：阳性率分别为８３３％、７４％和８１６％，并测定了３７例阳性脐静脉血中Ｇ－ＣＳＦ的含量，结果Ｓ／Ｎ最高１９９４，最低２１，ｘ±ｓ为５９±３９５。经统计学处理，脐静脉血和孕妇静脉血阳性率有显著性差异（Ｐ＜００１），脐静脉血和脐动脉血阳性率也有显著性差异（Ｐ＜００１）。并用单向环状免疫扩散（ＲＩＤ）法测得５１例正常足月儿脐静脉血中免疫球蛋白ＩｇＧ含量明显高于正常值（Ｐ＜００５）。结论：测得结果显示，脐血中Ｇ－ＣＳＦ主要来源于脐静脉的胎盘中。推测胎盘的某些细胞具有产生Ｇ－ＣＳＦ的功能。脐血中含有高水平的ＩｇＧ。
Objective: In order to find the site of G-CSF production in umbilical cord blood, to provide a scientific basis for the basic research and clinical application of umbilical cord blood. Methods: The positive rate of G-CSF in cord blood of 48 healthy pregnant women with gestational age between 37 and 38 weeks and 50 normal full-term neonates were measured by enzyme-linked immunosorbent assay using G-CSF ELISA kit. And content. Results: The positive rates were 833%, 74% and 816%, respectively. The content of G-CSF in 37 cases of positive umbilical vein blood was measured. The results showed that the highest S / N was 1994, the lowest was 21, x ± s is 59 ± 395. Statistically, the positive rate of venous blood in umbilical cord blood and pregnant women was significantly different (P <001), umbilical cord blood and umbilical artery blood rate was also significantly different (P <001). Immunoglobulin IgG levels in 51 cases of normal term umbilical blood were significantly higher than those of the normal group (P <005) by one-way cyclic immunodiffusion (RID). Conclusion: The measured results show that cord blood G-CSF mainly from the umbilical vein in the placenta. Some cells of the placenta are presumed to have the function of producing G-CSF. Cord blood contains high levels of IgG.