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目的 :探讨睡眠剥夺对大鼠学习能力的影响。方法 :采用小平台水环境法建立睡眠剥夺模型 ,以正常组和大平台组作为对照 ,检测大鼠海马一氧化氮 (NO)及一氧化氮合酶 (NOS)含量变化 ,并以Y型迷宫测量大鼠学习能力。结果 :(1)睡眠剥夺 1d、3d、5d组海马NO含量分别为(5 .2 6± 1.34)、(7.6 3± 1.86 )、(7.96± 2 .15 ) μmol·mgprot- 1,NOS含量分别为 (2 .4 6± 0 .6 8)、(2 .97± 0 .73)、(3.18± 0 .82 )U·mgprot- 1,分别高于正常对照组〔(3.4 9± 1.18) μmol·mgprot- 1、(1.32±0 .6 7)U·mgprot- 1〕和大平台组〔(4.35± 1.0 9) μmol·mgprot- 1、(1.89± 0 .6 2 )U·mgprot- 1(P <0 .0 1,0 .0 5 )〕 ;后两组比较无显著性差异。 (2 )睡眠剥夺 3d、5d组迷宫正确反应所需电击次数分别为 (2 8.1± 5 .2 )、(41.4± 7.9)次 ,所需时间为 (136 .4± 2 1.7)、(186 .7± 2 9.8)s ,高于正常对照组的 (2 2 .9± 4 .7)次 ,(10 9.6± 16 .5 )s和大平台组的 (16 .8± 3.5 )次 ,(82 .9± 13.2 )s(P <0 .0 1)。 (3)NO含量与迷宫正确反应所需电击次数及时间有显著正相关关系 (P <0 .0 1)。结论 :睡眠剥夺导致的大鼠学习能力下降可能与NO/NOS的神经毒性作用有关。
Objective: To investigate the effect of sleep deprivation on learning ability of rats. Methods: The sleep deprivation model was established by using the small platform water environment method. The changes of nitric oxide (NO) and nitric oxide synthase (NOS) in hippocampus of rats in normal group and large platform group were compared. Rat learning ability. Results: (1) NO concentrations in the hippocampus of the rats on sleep deprivation 1d, 3d and 5d were (5.26 ± 1.34), (7.63 ± 1.86) and (7.96 ± 2.15) μmol · mgprot-1 (2.46 ± 0.68), (2.97 ± 0.73) and (3.18 ± 0.82) U · mgprot-1 respectively, which were higher than that of the normal control group [(3.4 9 ± 1.18) μmol (1.32 ± 0.67) U · mgprot- 1] and the large plateau group [(4.35 ± 1.09) μmol · mgprot-1, (1.89 ± 0.62) U · mgprot- 1 <0. 0 1,0 .0 5)〕; after the two groups no significant difference. (2) The number of shocks required for sleep deprivation on 3d, 5d maze for correct response were (2 8.1 ± 5 .2) and (41.4 ± 7.9) times, respectively, and the time required was (136.4 ± 2 1.7) and (186) days. 7 ± 2 9.8 s, which was significantly higher than that of the control group (22.9 ± 4.7), (10 9.6 ± 16.5) s and (16.8 ± 3.5) s and (82. 9 ± 13.2) s (P <0.01). (3) There was a significant positive correlation between the content of NO and the number of shocks and the time required for proper maze response (P <0.01). Conclusion: The decreased learning ability induced by sleep deprivation may be related to the neurotoxic effect of NO / NOS.