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为探讨一氧化氮合酶与动脉粥样硬化斑块形成过程的相互关系 ,采用酶组织化学染色法和原位杂交法观察实验第 8周、12周和 16周三个阶段的兔主动脉粥样硬化斑块一氧化氮合酶活性及其 16周时的mRNA表达。结果显示 ,实验 8周时 ,主动脉斑块内一氧化氮合酶活性呈现弱阳性、阳性和强阳性三种不同的表现形式 ,并相应有不同的细胞分布方式 ;实验 12周时 ,一氧化氮合酶活性在斑块中广泛分布 ,且于中、内膜交界处更为明显 ;实验16周时 ,主动脉脂质斑块中一氧化氮合酶活性及其mRNA表达明显下降 ,此阶段的大面积mRNA表达阴性主要与脂质坏死中心的大量形成有关。提示动脉粥样硬化血管壁的一氧化氮合酶活性和基因表达是一个不断变化的动态过程 ,且因细胞类型和分布部位而异。
In order to explore the relationship between nitric oxide synthase and the formation of atherosclerotic plaque, the aortic intima of rabbits at three, eight and 16 weeks after the experiment was observed by enzyme histochemical staining and in situ hybridization Sclerosis plaque nitric oxide synthase activity and its mRNA expression at 16 weeks. The results showed that at 8 weeks, the activity of nitric oxide synthase in aortic plaque showed weak positive, positive and strong positive three different manifestations, and correspondingly different cell distribution; at 12 weeks of experiment, NOS activity in the plaque was widely distributed, and in the intima junction more obvious; experimental 16 weeks, the aortic lipid plaque nitric oxide synthase activity and its mRNA expression decreased significantly, this stage The negative expression of a large area of mRNA mainly associated with the massive formation of lipid necrosis centers. Suggesting that the activity of nitric oxide synthase and gene expression in the wall of atherosclerotic vessels is an ever-changing and dynamic process that varies depending on cell type and distribution site.