为明确死亡谷芽孢杆菌NBIF-001菌株对灰霉病的防治效果,采用平板对峙结合显微观察确定其对灰霉病菌的抑菌活性,并采用离体组织试验研究其对黄瓜叶部灰霉病及贮藏期番茄果实灰霉病的防治效果。结果表明,在平板对峙条件下NBIF-001对灰葡萄孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形;离体试验结果表明NBIF-001对灰霉病具有良好的生防效果,对黄瓜叶片灰霉病和番茄果实灰霉病的防效分别达到85.91%和72.52%。 In order to clarify the control effect of Bacillus cereus NBIF-001 against Botrytis cinerea, the antibacterial activity against Botrytis cinerea was determined by plate confrontation and microscopic observation. Prevention and Cure Effects of Tomato Fruit Gray Mold on Disease and Storage. The results showed that NBIF-001 had good antibacterial activity against Botrytis cinerea in the condition of plate confrontation. The hypha strain, swelling and deformation were observed under the microscope. The results of in vitro experiments showed that NBIF-001 had good antibacterial activity against Botrytis cinerea The biocontrol effect was 85.91% and 72.52% for cucumber leaf gray mold and tomato fruit gray mold respectively.