主要研究注模前酶作用时间对TGase改性的三种大豆分离蛋白膜(SPI-1膜、SPI-2膜及SPI-3膜)的抗拉强度和断裂伸长率的影响。研究表明酶作用时间60min以内,TGase的作用增加了抗拉强度。这可能是因为TGase使蛋白质分子中产生了共价交联从而形成高分子聚合物的缘故。而酶作用时间60min以内,EB值的变化各有不同。这是底物和酶作用时间两大因素相互作用的结果。在制备成膜溶液时,适度均质及超声可增加TGase改性膜的机械性能。 The effects of enzyme action time on the tensile strength and elongation at break of three soybean protein isolate films (SPI-1, SPI-2 and SPI-3) modified by TGase were studied. Studies have shown that within 60min enzyme action time, the role of TGase increased tensile strength. This may be due to the fact that TGase forms covalently crosslinked protein molecules to form high molecular weight polymers. The enzyme action time within 60min, EB values ​​vary. This is the result of interaction between the substrate and the enzyme’s action time. In the preparation of film-forming solution, moderate homogenization and ultrasound can increase the TGase modified film mechanical properties.