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从华北高粱红条病叶中得到一种病毒分离物.此病毒侵染玉米、谷子及禾草产生花叶症状,在多年生寄主矛叶荩草(Arthraxon lanceolatus)、大油芒(Spodiopogon Sibiricus)、牛鞭草(HemarthriaCompressa)上越冬,在田间由麦二叉蚜(Schizaphis graminum)、粟缢管蚜(Rhopalosiphum Padi)、玉米蚜(R.maidis)、桃蚜(Myzus peisicae)等以非持久性方式传播,汁液摩擦可传毒。高粱品种954066B的种子带毒率在本实验中为0.03%。病毒粒体线条状,略弯曲;长度为750nm,直径13nm,A260/A280比值为1.22,衣壳亚基蛋白分子量约为36,400道尔顿.在高粱汁液中,钝化温度为54℃,稀释限点为10~(-3),体外存活期3天.此病毒与甘蔗花叶病毒(SCMV)及玉米矮花叶病毒(MDMV)均有血清反应关系;不侵染约翰逊草(Sorghum halepense)及甘蔗(Saccharum officinarum),结合其他特性确定华北高粱红条病毒病是由玉米矮花叶病毒B株系(MDMV-—B)所致.
A virus isolate was isolated from the leaves of the red stripe of Sorghum bicolor in North China.The virus infects maize, millet and grasses and produces mosaic symptoms, which are commonly found in perennial arthropods such as Arthraxon lanceolatus, Spodiopogon Sibiricus, Winter wintering on Hemarthria Compressa occurs in the field in a non-persistent manner by the Schizaphis graminum, Rhopalosiphum Padi, R. maidis, Myzus peisicae, Spread, juice friction can be poisoned. Sorghum variety 954066B was 0.03% seed-borne in this experiment. The virion was linear and slightly curved with a length of 750 nm and a diameter of 13 nm, with A260 / A280 ratio of 1.22 and capsid subunit protein of about 36,400 Dalton. In sorghum juice, the passivation temperature was 54 ° C and the dilution limit Point was 10 -3, and the survival time in vitro was 3 days.This virus had a seroprevalence relationship with both SCMV and MDMV, and did not invade Sorghum halepense and Sugarcane (Saccharum officinarum), combined with other traits, was used to determine the susceptibility of North China sorghum stripe virus to maize dwarf mosaic virus B (MDMV - B).