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目的:建立超高效液相色谱法测定一捻金中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量的方法。方法:色谱柱:ZORBAX Eclipse Plus C18柱(2.1 mm×50 mm,1.8μm);流速:0.21 ml·min-1;柱温:30℃;流动相:乙腈-水梯度洗脱;检测波长:203 nm;进样量:1μl。结果:人参皂苷Rg1在浓度为0.020 3~0.303 9 mg·ml-1时线性关系良好(r=0.999 6),平均回收率为99.05%,RSD为1.3%(n=6);人参皂苷Re在浓度为0.020 2~0.302 7 mg·ml-1时线性关系良好(r=0.999 8),平均回收率为101.31%,RSD为1.1%(n=6);人参皂苷Rb1在浓度为0.020 3~0.305 1 mg·ml-1时线性关系良好,r=0.999 8,平均回收率为100.71%,RSD为0.9%(n=6)。结论:该方法简单、准确,可同时测定3种人参皂苷的含量,可作为一捻金的质量控制。
OBJECTIVE: To establish a method for the determination of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Radix Astragali using ultra performance liquid chromatography. Method: Column: ZORBAX Eclipse Plus C18 column (2.1 mm × 50 mm, 1.8 μm); flow rate: 0.21 ml · min -1; column temperature: 30 ℃; mobile phase: acetonitrile- nm; injection volume: 1μl. Results: The ginsenoside Rg1 had a good linearity (r = 0.999 6) with an average recovery of 99.05% and a RSD of 1.3% (n = 6) at a concentration of 0.020 3-0.303 9 mg · ml-1. The average recovery was 101.31% and the RSD was 1.1% (n = 6) at a concentration of 0.020 2-0.302 7 mg · ml-1. The concentration of ginsenoside Rb1 at a concentration of 0.020 3-0.305 The linearity was good at 1 mg · ml-1, r = 0.999 8, with an average recovery of 100.71% and a RSD of 0.9% (n = 6). Conclusion: The method is simple and accurate, and can simultaneously determine the content of three kinds of ginsenosides, which can be used as the quality control of twisting gold.