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目的:了解厦门、深圳及广州的鼠形动物脑组织标本基孔肯雅病毒(CHIKV)的携带情况,分析鼠形动物作为人类CHIKV宿主和潜在传染源的可能性。方法:选取2013年1月至2016年6月在厦门、深圳及广州(白云区、越秀区)部分居民区、城市公园、医院、农贸市场、学校捕获鼠形动物,无菌采集其脑组织样本,用逆转录聚合酶链反应(RT-PCR)方法检测CHIKV。结果:本次调查共捕获鼠形动物1 092只2目2科3属7种,家鼠以褐家鼠为优势鼠种,野鼠以黄毛鼠为优势鼠种,共获1 092份脑组织标本,RT-PCR方法未在采集的脑组织标本中检测出CHIKV。结论:厦门、深圳及广州鼠形动物作为CHIKV宿主和传染源的可能性小。
OBJECTIVE: To understand the carriage of chikungunya virus (CHIKV) from rat brain samples in Xiamen, Shenzhen and Guangzhou, and to analyze the possibility of rat as CHIKV host and potential source of infection. METHODS: From January 2013 to June 2016, murine animals were collected from some residential areas, urban parks, hospitals, farmer’s markets and schools in Xiamen, Shenzhen and Guangzhou (Baiyun District and Yuexiu District), and their brain tissue samples were collected aseptically , CHIKV was detected by reverse transcription polymerase chain reaction (RT-PCR). Results: A total of 1 092 species of 2 species, 2 families, 2 families, 3 genera and 7 species of rat were captured in this investigation. Rattus norvegicus was the dominant species in house mouse, Specimens, RT-PCR method did not detect CHIKV in the brain tissue samples collected. Conclusion: There is little possibility that mouse-like animals in Xiamen, Shenzhen and Guangzhou may be used as CHIKV hosts and sources of infection.