用Trsger等报道的恶性疟原虫体外培养方法常规培养疟原虫,原虫率很少超过10～15%,且新分离株对这一培养系统的适应能力也很不一致。为了增进恶性疟原虫体外生长,本文作者报道了一种用肝细胞作为营养层培养恶性疟原虫的新方法。这种方法为:用胶原蛋白酶溶液灌注瑞士小鼠肝脏,获得肝细胞,将肝细胞与肝上皮细胞共同孵育于含10%人脐带血清的RPMI1640培养液中,再加入含虫血,按常规置蜡烛缸内进行体外培养。 Plasmodium falciparum was routinely cultured in vitro using P. falciparum as reported by Trsger et al. The parasite rate rarely exceeded 10-15% and the new isolates were also not well adapted to this culture system. To enhance the in vitro growth of P. falciparum, the authors report a new method of culturing P. falciparum using hepatocytes as a nutrient layer. In this method, the liver of the Swiss mouse is perfused with the collagenase solution, and the hepatocytes are incubated with the liver epithelial cells in RPMI1640 medium containing 10% human umbilical cord serum. Candle tank for in vitro culture.